Identification of closely related Agrobacterium vitis isolates by chromosomal DNA probes.
Identifieur interne : 000A21 ( Main/Exploration ); précédent : 000A20; suivant : 000A22Identification of closely related Agrobacterium vitis isolates by chromosomal DNA probes.
Auteurs : L. Otten [France] ; J. CanadaySource :
- Microbial releases : viruses, bacteria, fungi [ 0940-9653 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), Cartographie chromosomique (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Famille multigénique (MeSH), Gènes bactériens (MeSH), Plasmides (MeSH), Polymorphisme génétique (MeSH), Rhizobium (classification), Rhizobium (génétique), Similitude de séquences d'acides nucléiques (MeSH), Sondes d'ADN (MeSH), Séquence nucléotidique (MeSH), Séquences répétées d'acides nucléiques (MeSH).
- MESH :
- analyse : ADN bactérien.
- génétique : Rhizobium.
- Cartographie chromosomique, Clonage moléculaire, Données de séquences moléculaires, Famille multigénique, Gènes bactériens, Plasmides, Polymorphisme génétique, Rhizobium, Similitude de séquences d'acides nucléiques, Sondes d'ADN, Séquence nucléotidique, Séquences répétées d'acides nucléiques.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Chromosome Mapping (MeSH), Cloning, Molecular (MeSH), DNA Probes (MeSH), DNA, Bacterial (analysis), Genes, Bacterial (MeSH), Molecular Sequence Data (MeSH), Multigene Family (MeSH), Plasmids (MeSH), Polymorphism, Genetic (MeSH), Repetitive Sequences, Nucleic Acid (MeSH), Rhizobium (classification), Rhizobium (genetics), Sequence Homology, Nucleic Acid (MeSH).
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical : DNA Probes.
- classification : Rhizobium.
- genetics : Rhizobium.
- Base Sequence, Chromosome Mapping, Cloning, Molecular, Genes, Bacterial, Molecular Sequence Data, Multigene Family, Plasmids, Polymorphism, Genetic, Repetitive Sequences, Nucleic Acid, Sequence Homology, Nucleic Acid.
Abstract
Crown gall formation on grapevine by Agrobacterium vitis is an important plant disease in many regions of the world. On grapevine, octopine/cucumopine (o/c) strains are widely distributed. Here we describe two chromosomal sequences of o/c strains of 15 and 20 kb, which reveal a high degree of polymorphism in different o/c isolates. Part of the polymorphism is due to the presence or absence of a 2.2-kb-long repeated sequence, a homolog of which is also found on the octopine Ti plasmid of Ach5, immediately to the left of the left TL-region border. The occurrence and distribution of this repeat in different o/c isolates make it possible to reconstruct the evolution of these strains. The chromosomal DNA sequences outside the repeats also differ in various isolates. The two probes described here can be used to identify strains from a collection, classify new isolates, or trace a given isolate under experimental release conditions.
PubMed: 1341986
Affiliations:
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Le document en format XML
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Otten</name><affiliation wicri:level="3"><nlm:affiliation>Plant Molecular Biology Institute of the C.N.R.S., Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Plant Molecular Biology Institute of the C.N.R.S., Strasbourg</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Canaday, J" sort="Canaday, J" uniqKey="Canaday J" first="J" last="Canaday">J. 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On grapevine, octopine/cucumopine (o/c) strains are widely distributed. Here we describe two chromosomal sequences of o/c strains of 15 and 20 kb, which reveal a high degree of polymorphism in different o/c isolates. Part of the polymorphism is due to the presence or absence of a 2.2-kb-long repeated sequence, a homolog of which is also found on the octopine Ti plasmid of Ach5, immediately to the left of the left TL-region border. The occurrence and distribution of this repeat in different o/c isolates make it possible to reconstruct the evolution of these strains. The chromosomal DNA sequences outside the repeats also differ in various isolates. The two probes described here can be used to identify strains from a collection, classify new isolates, or trace a given isolate under experimental release conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1341986</PMID><DateCompleted><Year>1994</Year><Month>02</Month><Day>03</Day></DateCompleted><DateRevised><Year>2006</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0940-9653</ISSN><JournalIssue CitedMedium="Print"><Volume>1</Volume><Issue>1</Issue><PubDate><Year>1992</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Microbial releases : viruses, bacteria, fungi</Title><ISOAbbreviation>Microb Releases</ISOAbbreviation></Journal><ArticleTitle>Identification of closely related Agrobacterium vitis isolates by chromosomal DNA probes.</ArticleTitle><Pagination><MedlinePgn>29-34</MedlinePgn></Pagination><Abstract><AbstractText>Crown gall formation on grapevine by Agrobacterium vitis is an important plant disease in many regions of the world. On grapevine, octopine/cucumopine (o/c) strains are widely distributed. Here we describe two chromosomal sequences of o/c strains of 15 and 20 kb, which reveal a high degree of polymorphism in different o/c isolates. Part of the polymorphism is due to the presence or absence of a 2.2-kb-long repeated sequence, a homolog of which is also found on the octopine Ti plasmid of Ach5, immediately to the left of the left TL-region border. The occurrence and distribution of this repeat in different o/c isolates make it possible to reconstruct the evolution of these strains. The chromosomal DNA sequences outside the repeats also differ in various isolates. The two probes described here can be used to identify strains from a collection, classify new isolates, or trace a given isolate under experimental release conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Otten</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Plant Molecular Biology Institute of the C.N.R.S., Strasbourg, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Canaday</LastName><ForeName>J</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Microb Releases</MedlineTA><NlmUniqueID>9301294</NlmUniqueID><ISSNLinking>0940-9653</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015342" MajorTopicYN="N">DNA Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="N">Genes, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="N">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="N">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012091" MajorTopicYN="N">Repetitive Sequences, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1341986</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Strasbourg</li></settlement></list><tree><noCountry><name sortKey="Canaday, J" sort="Canaday, J" uniqKey="Canaday J" first="J" last="Canaday">J. Canaday</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Otten, L" sort="Otten, L" uniqKey="Otten L" first="L" last="Otten">L. Otten</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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